Feasibility and Acceptability of a Social Media-Based Physical Activity Intervention for College Women

Psychology of sport and exercise · 2026-05-01

The relationship between internalized weight stigma and acceptance of weight-loss treatment resources

Journal of Health Psychology · 2026-01-30

= 157) reported on IWS, weight-loss desire, eating self-efficacy (ESE), and treatment preferences. Participants expressing weight-loss desire were offered weight-loss treatment resources and asked to accept/decline them. Binary logistic regression examined whether IWS was associated with accepting weight-loss treatment resources, and ESE as a moderator. Exploratory analyses tested associations between IWS and treatment preferences. Participants with higher (vs lower) IWS had significantly higher odds of accepting weight-loss treatment resources (OR = 1.52, 95% CI: [1.12-2.05]). ESE did not moderate this relationship. In exploratory analyses, higher IWS was associated with greater interest in psychological intervention for IWS combined with weight-loss treatment. IWS was not associated with most preferences (e.g. group vs individual or in-person vs video sessions) when accounting for weight-loss desire. Further research should clarify how IWS affects treatment acceptance.

Structure-Activity Relationship Studies Towards Analogues of Pleconaril as Novel Enterovirus-D68 Capsid-Targeting Antivirals

bioRxiv (Cold Spring Harbor Laboratory) · 2025-08-11

Non-polio enteroviruses (NPEV) such as enterovirus D68 (EV-D68) that are highly infectious and associated with polio-like neurological complications have caused outbreaks, globally, in recent years. While some clinical and preclinical compounds have shown efficacy against NPEV in-vitro, liabilities that caused historical compounds such as pleconaril to fall short of FDA approval still remain. We present herein SAR and SPR studies of analogues of clinical compounds such as pleconaril and vapendavir against EV-D68 as a representative NPEV. Numerous structurally differentiated analogues with EV-D68 antiviral activity and useful ADME properties were discovered, which could serve as starting points for future EV drug discovery campaigns. Screening against a panel of enteroviruses revealed moderately broad-spectrum anti-EV activity of compound 26.

Live Virus EV-D68 - RD - Antiviral Screening Assay v1

2025-04-18

The following is a protocol for live virus antiviral screening against EV-D68 in vitro using Rhabdomyosarcoma (RD) cells. RD Cells are prophylactically treated with antiviral candidates that have been serially diluted to investigate activity, via IC50 values generated via analysis of immunofluorescent staining, against EV-D68. You may opt to include PgP inhibitor in the assay by adding 2uM into the infection media. Each live virus screening assay is accompanied by a matching cytotoxicity screening assay in uninfected cells to investigate drug toxicity.

How Weight Bias and Stigma Undermine Healthcare Access and Utilization

Current Obesity Reports · 2025-01-20 · 18 citations

An mRNA-based broad-spectrum antiviral inspired by ISG15 deficiency protects against viral infections in vitro and in vivo

Science Translational Medicine · 2025-08-13 · 6 citations

Type I interferons (IFN-Is) are cytokines with potent antiviral and inflammatory capacities. IFN-I signaling drives the expression of thousands of IFN-I-stimulated genes (ISGs), whose aggregate function results in the control of viral infections. A few of these ISGs are tasked with negatively regulating the IFN-I response to prevent overt inflammation. ISG15 is a negative regulator whose absence leads to persistent, low-grade elevation of ISG expression and concurrent, often self-resolving, mild autoinflammation. The limited breadth and low-grade persistence of ISGs expressed in ISG15 deficiency are sufficient to confer broad-spectrum antiviral resistance. Inspired by the antiviral state of humans with ISG15 deficiency, we identified a nominal collection of 10 ISGs that recapitulated the broad antiviral potential of the IFN-I system, which typically induces the expression of thousands of ISGs. The expression of this 10-ISG collection in an IFN-I-nonresponsive cell line increased cellular resistance to Zika virus, vesicular stomatitis virus, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A lipid nanoparticle-encapsulated messenger RNA (mRNA) formulation of this 10-ISG collection reduced influenza A virus plaque size in samples collected from infected mice when given prophylactically. Moreover, when used collectively and delivered prophylactically, the 10-ISG collection was able to protect hamsters against a lethal SARS-CoV-2 challenge, in contrast with the lack of efficacy when mRNAs were delivered individually. These findings suggest that these 10 ISGs have potential as a broad-spectrum antiviral prophylactic.

Internalized weight stigma, metabolic syndrome, and inflammation in postmenopausal women with obesity

Brain Behavior & Immunity - Health · 2025-11-01

Objective: To determine the relationship of internalized weight stigma with metabolic syndrome (MetS) and markers of inflammation. Methods: = 101) with high or low scores on the Weight Bias Internalization Scale completed a single assessment visit. MetS components (waist circumference, blood pressure, triglycerides, HDL cholesterol, and glucose) were measured, along with body mass index (BMI). Blood samples were drawn to analyze C-reactive protein, interleukin-6, and myeloperoxidase. Participants completed a second measure of internalized weight stigma (Weight Self-Stigma Questionnaire; WSSQ) and reported medications, demographics, depression symptoms, smoking status, and perceived stress (included as covariates). Results: = 0.030). Both measures of internalized weight stigma were associated with greater continuous blood pressure values. Inflammatory markers were not associated with internalized weight stigma in most analyses. Conclusions: Some significant associations were found between internalized weight stigma and MetS components, but more research is needed to clarify these relationships.

The impact of patient weight on US mental health providers’ diagnosis of bulimia nervosa

Eating Disorders · 2025-02-16

s < .01). These findings suggest poor detection of BN among mental health providers when patients present with healthy or higher weights. Providers may benefit from improved training for detecting BN when excessive exercise is used as the primary compensatory behavior.

Weight Bias Internalization Scale-Modified (WBIS-M)

Cambridge University Press eBooks · 2025-11-20

Bioluminescent reporter influenza A viruses to track viral infections

bioRxiv (Cold Spring Harbor Laboratory) · 2025-07-15

ABSTRACT Influenza A viruses (IAV) infect a wide range of mammal and bird species and are responsible for seasonal outbreaks and occasional pandemics. Studying IAV requires methods to detect the presence of the virus in infected cells or animal models. Recombinant IAV expressing fluorescent proteins have allowed monitoring viral infection in cultured cells and ex vivo in the organs of infected animals. However, fluorescent-expressing IAV are often attenuated and are not suited for the imaging of infected animals using in vivo imaging systems (IVIS). To overcome this limitation, we generated a recombinant A/California/04/2009 H1N1 (pH1N1) expressing nanoluciferase (Nluc) from the non-structural (NS) viral segment (pH1N1-Nluc) that replicates efficiently in vitro , with growth kinetics and plaque morphology comparable to wild-type pH1N1 (pH1N1-WT). We used this pH1N1-Nluc to demonstrate its ability to identify neutralizing antibodies and antivirals, with neutralization and inhibition results comparable to pH1N1-WT. In mice, pH1N1-Nluc was able to induce similar body weight loss and mortality, and viral titers comparable to pH1N1-WT, results that were recapitulated in a ferret model of IAV infection. Using IVIS, pH1N1-Nluc enabled non-invasive, real-time tracking of viral infection in vivo and ex vivo following infection of mice with viral titers comparable to pH1N1-WT. The flexibility of this approach was further demonstrated by the generation of a Nluc-expressing recombinant A/Puerto Rico/8/1934 H1N1 (PR8-Nluc). Altogether, our results demonstrate that Nluc-expressing recombinant IAV represent a valuable tool for in vitro and in vivo studies, including the identification of antivirals and/or neutralizing antibodies, and to assess protective efficacy of vaccines. IMPORTANCE Despite the availability of recombinant influenza A viruses (IAV) expressing fluorescent reporter genes to track viral infections in vitro and ex vivo , these viruses are often attenuated and do not represent the best option for imaging entire animals using in vivo imaging systems (IVIS). To solve this limitation, we generated recombinant influenza pandemic A/California/04/2009 H1N1 expressing nanoluciferase (pH1N1-Nluc) from the viral non-structural (NS) segment and demonstrate how expression of Nluc does not affect viral replication in vitro or viral pathogenesis in vivo. Importantly, we demonstrate the feasibility of detecting pH1N1-Nluc infection in vivo using IVIS. We also validate the flexibility of this approach by generating an influenza A/Puerto Rico/8/1934 H1N1 (PR8-Nluc). Our results support the feasibility of using these recombinant IAVs expressing Nluc from the NS segment for in vitro and in vivo studies, including the identification of neutralizing antibodies and/or antivirals, and to assess protective efficacy of vaccines.