About

Dr. Alana Dasgupta is a faculty member associated with the AI4Path Ohio State research team. The provided page text does not contain specific details about her research focus, background, or key contributions. Therefore, a detailed biography cannot be generated from the available information.

Research topics

• Medicine
• Internal medicine
• Gastroenterology
• Microbiology
• Endocrinology
• Biology
• Chemistry
• Biochemistry
• Surgery
• Immunology
• Virology
• Urology
• Pathology

Selected publications

• Changes in the Glomerular Basement Membrane Thickness in Living-Donor Baseline Biopsies Within a 15-Year Period

  Journal of the American Society of Nephrology · 2025-10-01

  article
  PublisherDOI

• Utility of Different Anti-IgG Antibodies in Recognition of Punctate (Powdery) Podocyte Staining by Immunofluorescence in Patients with Diffuse Podocyte Foot Process Effacement

  2025-11-07

  articleOpen access1st authorCorresponding

  Circulating anti-nephrin autoantibodies recently were recognized as a possible pathogenic factor in patients with steroid-resistant minimal change disease. In a kidney biopsy, these patients have punctate ("powdery") staining in the podocytes by immunofluorescence. Previously published data described such pattern of staining by using anti-IgG Dako antibody. In the routine practice, different laboratories use different anti-IgG antibodies from a variety of vendors. The aim of this work was to compare the ability to recognize the punctate staining in the podocyte by two different anti-IgG antibodies (Dako and Kent) that are in use in our routine renal pathology practice. Our data indicates that both anti-IgG antibodies recognize the punctate ("powdery) staining in the podocytes in a subset of patients with minimal change disease.

  PublisherOA PDFDOI

• Proteinuria and proximal tubular epithelial cells: correlation between immunofluorescence, histology, and degree of proteinuria

  Frontiers in Nephrology · 2024-10-31 · 1 citations

  articleOpen access

  Proteins are filtered from the blood through the glomerular filtration barrier. Filtered proteins are reabsorbed by proximal tubular epithelial cells (PTECs), which have been shown to possess the ability to regulate protein reabsorption. Histologically, these reabsorbed proteins are seen as tubular protein reabsorption droplets (TPRDs). Experimental studies indicate that PTECs play an important role in regulating proteinuria but the correlations between TPRD and the degree of proteinuria in human kidney biopsies have not been investigated in detail. Consecutive native kidney biopsies with non-proliferative glomerular disease performed at the OSUWMC for a 1-year period were analyzed. Cases with acute glomerular diseases and inadequate biopsies were excluded. The staining intensity and the percentage of TPRDs, as well as other morphologic parameters, were assessed. A total of 109 kidney biopsies were included in the study. A reverse correlation was identified between the percentage of albumin TPRDs and proteinuria ( p = 0.047). There were positive correlations between proteinuria and the staining intensity for IgG TPRDs ( p = 0.05) and the degree of acute tubular necrosis (ATN) ( p = 0.015). In patients with no ATN, positive correlations between proteinuria and albumin and IgG TPRDs were seen, whereas in patients with ATN, these correlations were lost. A positive correlation was seen between proteinuria and chronic kidney injury. A strong correlation was noted between the degree of proteinuria and podocyte foot process effacement. Our data indicate that PTECs regulate proteinuria by absorbing proteins from the urine filtrate. Therefore, based on the human renal biopsy material, our study confirms that well-functioning renal PTECs play an important role in the regulation of proteinuria.

  PublisherOA PDFDOI

• A Cat and a "Full House"

  Journal of the American Society of Nephrology · 2024-10-01

  article

  Introduction: In a patient with constitutional symptoms, positive ANA serologies, and hypocomplementemia, immune complex glomerulonephritis (GN) often suggests lupus nephritis. However, Bartonella endocarditis can mimic this presentation. We detail the case of a 34-year-old man with history of pulmonary valve replacement and cat bite with acute kidney injury (AKI) due to Bartonella endocarditis-related GN. Case Description: The patient initially presented with upper respiratory symptoms, diarrhea, and AKI, attributed to hypovolemia and NSAID use. AKI worsened despite resolution of symptoms. Further workup revealed elevated rheumatoid factor, positive Epstein-Barr Virus (EBV) IgM, normal complement levels, and negative infectious workup. Kidney biopsy was consistent with IgA nephropathy. Post-discharge, he developed a pruritic rash, assumed to be IgA vasculitis. Twelve days later, he returned with a new pulmonic regurgitation murmur, weight loss, nocturnal fevers, and heart failure symptoms. Both transthoracic and transesophageal echocardiograms were negative for vegetations, but Bartonella IgG titers were elevated. Glucocorticoids resolved symptoms and AKI temporarily, but they recurred post-tapering, prompting transfer to our institution. Repeat serologies showed positive ANCA, low C3 and C4, and high Bartonella titers. Repeat kidney biopsy revealed mesangioproliferative GN with full-house immune complex deposition in the mesangium and also in the subendothelial, subepithelial and intramembranous spaces. Bartonella DNA was detected, leading to hospital readmission for bacteremia and pulmonic valve vegetation. He was treated with antibiotics leading to and then following valve replacement. Discussion: The differential diagnosis for immune complex glomerulonephritis includes autoimmune disease, malignancy and infection which may often be missed at the time of acute infection due to mild symptoms and a negative infectious workup due to a fastidious and/or slow-growing microbe. Bartonella is most often diagnosed in the later stages of infection with elevated IgG titers rather than IgM and PCR as it is a facultative intracellular bacterium, rarely isolated in culture and only fleetingly found in the blood. When the kidney biopsy shows an immune complex glomerulonephritis, always consider smoldering infections, especially if the patient is immunocompromised or has a history of cardiac valve surgery.

  PublisherDOI

• WCN24-1802 Outcomes in patients with anticoagulant related nephropathy: from bad to worse

  Kidney International Reports · 2024-04-01

  articleOpen access

  Introduction: Methods: Results: I have potential conflict of interest to disclose.

  PublisherOA PDFDOI

• Clinicopathological differences between Bartonella and other bacterial endocarditis-related glomerulonephritis – our experience and a pooled analysis

  Frontiers in Nephrology · 2024 · 14 citations

  • Pathology
  • Medicine
  • Biology

  Background: spp. has concomitantly emerged as the leading cause of "blood culture-negative IE" (BCNE). Pre-disposing factors, clinical presentation and kidney biopsy findings in Bartonella IE-associated glomerulonephritis (GN) show subtle differences and some unique features relative to other bacterial infection-related GNs. We highlight these features along with key diagnostic clues and management approach in Bartonella IE-associated GN. Methods: We conducted a pooled analysis of 89 cases of Bartonella IE-associated GN (54 published case reports and case series; 18 published conference abstracts identified using an English literature search of several commonly used literature search modalities); and four unpublished cases from our institution. Results: spp. early in the course of management can help in early diagnosis and initiation of appropriate treatment. Conclusion: Diagnosis of IE-associated GN can be challenging particularly with BCNE. The number of Bartonella IE-associated GN cases in a single institution tends to be less than IE due to gram positive cocci, however Bartonella is currently the leading cause of BCNE. We provide a much-needed discussion on this topic.

  PublisherOA PDFDOI

• Role of plasminogen activated inhibitor-1 in the pathogenesis of anticoagulant related nephropathy

  Frontiers in Nephrology · 2024-06-28

  articleOpen access

  Anticoagulant related nephropathy (ARN) is the result of glomerular hemorrhage in patients on systemic anticoagulation therapy or underlying coagulopathy. Red blood cells (RBC) that passed through the glomerular filtration barrier form RBC casts in the tubules, increase oxidative stress and result in acute tubular necrosis (ATN). The mechanisms of ARN still not completely discovered. Plasminogen activator inhibitor-1 (PAI-1) plays a significant role in the maintenance of coagulation homeostasis. We developed an animal model to study ARN in 5/6 nephrectomy (5/6NE) rats. The aim of this study was to elucidate the role of PAI-1 in the ARN pathogenesis. 5/6NE rats were treated per os with warfarin (0.75 mg/kg/day) or dabigatran (150 mg/kg/day) alone or in combination with PAI-1 antagonist TM5441 (2.5, 5.0 and 10 mg/kg/day). TM5441 in a dose dependent manner ameliorated anticoagulant-induced increase in serum creatinine in 5/6NE rats. Anticoagulant-associated increase in hematuria was no affected by TM5441. The levels of reactive oxygen species (ROS) in the kidneys were in a dose-dependent manner decreased in 5/6NE rats treated with an anticoagulant and TM5441. Our data demonstrates that PAI-1 may reduce ARN by decreasing ROS in the kidneys. Glomerular hemorrhage is not affected by anti-PAI-1 treatment. These findings indicate that while symptoms of ARN can be reduced by PAI-1 inhibition, the main pathogenesis of ARN - glomerular hemorrhage - cannot be prevented.

  PublisherOA PDFDOI

• WCN24-1960 Membranous glomerulonephritis with unusual C3 deposits: case series report

  Kidney International Reports · 2024-04-01

  articleOpen access

  Introduction: Methods: Results: We present an unusual cases of MN that showed some features of primary MN, but also features resembling C3 glomerulonephritis. None of the patients had underlying autoimmune disease, monoclonal gammopathy or recent infection. All patients either had COVID-19 infection or were vaccinated against it. Thus, COVID-19 infection or vaccination could modulate immune system, resulting in an unusual presentation of MN.

  PublisherOA PDFDOI

• Kidney Biopsy Corner: Amyloidosis

  Glomerular Diseases · 2023-09-08 · 4 citations

  articleOpen access

  Amyloidosis is an infiltrative disease caused by misfolded proteins depositing in tissues. Amyloid infiltrates the kidney in several patterns. There are, as currently described by the International Society of Amyloidosis, 14 types of amyloid that can involve the kidney, and these types may have different locations or clinical settings. Herein we report a case of AA amyloidosis occurring in a 24-year-old male with a history of intravenous drug abuse and provide a comprehensive review of different types of amyloids involving the kidney.

  PublisherDOI

• N-Acetylcysteine Ameliorates Hematuria-Associated Tubulointerstitial Injury in 5/6 Nephrectomy Mice

  Journal of the American Society of Nephrology · 2023-11-01

  article
  PublisherDOI

Frequent coauthors

• Laura Biederman

  Nationwide Children's Hospital

  15 shared

• Anjali A. Satoskar

  The Ohio State University

  10 shared

• Sergey V. Brodsky

  The Ohio State University Wexner Medical Center

  9 shared

• Tibor Nádasdy

  6 shared

• Galina Mikhalina

  Regional Health

  5 shared

• Mineaki Kitamura

  Nagasaki Institute of Applied Science

  4 shared

• Mohankumar Doraiswamy

  Cooper Green Mercy Hospital

  4 shared

• Edward A. Clark

  St. Vincent Hospital

  3 shared

Labs

• Computational Pathology LabPI