About

Dan Fraenkel is not explicitly described in the provided page text, which primarily focuses on the history, structure, and celebration of Harvard’s Biochemical Sciences Tutorial Program. The text mentions faculty members such as Stephen C. Harrison and others involved in guiding and supporting the program, but does not provide specific information about Dan Fraenkel’s research focus, background, or key contributions.

Research topics

• Political Science
• Medicine
• Virology
• Psychology
• Medical education
• Physics
• Law
• Internal medicine

Selected publications

• Testing for the Virus in the First Year of COVID-19: A Primer and a Plea for the Economists

  Challenge · 2021

  1st authorCorresponding
  • Political Science
  • Virology
  • Law

  The author addresses practice and uncertainties in testing for infections.

  PublisherDOI

• Corrective Metabolism: An Appreciation

  2019-06-20

  article1st authorCorresponding
  Publisher

• Involvement of kinases in glucose X Saccharomyces cerevisiae

  2016-01-01

  articleSenior author

  Uptake of glucose, fructose, and the nonmetabo- lizable analog 6-deoxyglucose was measured in wild-type Saccha- romyces cerevisiae and two mutant strains, one (hxkl hxk2) lacking both hexokinase A (P-I) and B (P-II) but containing glucokinase (and hence able to grow on glucose but not fructose) and the other (hxkl hxk2 glk) also lacking glucokinase (and not able to grow on glucose either). Uptake of the nonmetabolized substances (i.e., 6-deoxy- glucose in all three strains, fructose in the two mutants, and glu- cose in the triple mutant) reached a plateau at or below the ex- ternal concentration. The kinetic characteristics of were determined from 5-sec incubations by plotting velocity (V) vs. ve- locity/substrate concentration (V/S) curves. According to such plots, in the wild-type strain had two components, affinity uptake with Km values of ca. 1 mM for glucose and 6 mM for fruc- tose and affinity uptake with Km values of ca. 20 and 50 mM, respectively. The double kinase mutant showed both components for glucose but only the high Km component for fructose, while the triple kinase mutant showed only high Km for both glucose and fructose. Genetic analysis showed that only in strains lacking both hexokinases (hxkl hxk2) was the low Km system for fructose absent. Low Km was restored to the triple mutant by in- troduction of the cloned wild-type genes: HXK1 or HXK2, for fruc- tose uptake, and HXKI, HXK2, or GLK1, for glucose uptake. A phosphoglucose isomerase mutant had both low and high Km up- take for glucose. These results indicate the presence of two types of mechanism for glucose and fructose in yeast, the func- tioning of one of which, the low Km system, is influenced by the cognate kinases.

  Publisher

• Trehalose-6-phosphate synthase and stabilization of yeast glycolysis

  FEMS Yeast Research · 2015-11-11 · 8 citations

  article1st authorCorresponding

  ‘Lost in transition: Startup of glycolysis yields subpopulations of nongrowing cells…’ (‘LIT’, van Heerden et al. 2014) is a massive paper from groups in Amsterdam and Delft, which deals with broad issues in metabolism and cell heterogeneity, as addressed for the predominant metabolic pathway, glycolysis, in the context of a long studied but incompletely understood yeast mutant which is impaired in use of glucose without evident direct defects in the pathway. The primary approach is the quite original one of predicting, for the mutant, the dynamics of metabolism upon glucose addition, based on a mathematical model using the known kinetics for the enzymes of the pathway. Here we will discuss this paper and provide some additional model simulations illuminating the model.

  PublisherDOI

• Yeast Intermediary Metabolism

  2011-05-10 · 35 citations

  book1st authorCorresponding

  The intermediary metabolism of small molecules is the meat and potatoes of cell function. The pathways and modes of obtaining energy, degradation and utilization of exogenous organic nutrients, and formation of the building blocks of the main macromolecules were a major focus of research in biology from the turn of the 20th century into the 1970s. Other matters have come to prominence, but the field is active, with interesting problems that are central to biology and medicine. Molecular biology developed through the use of one bacterium, Eschericha coli, with the saying What's true for E. coli is true for elephants. In recent years, an analogous workhorse has been the eukaryotic microbe baker's yeast, Saccharomyces cerevisiae, used in many studies of cell biology common to multicellular organisms. This single-volume handbook explains metabolism as based on Saccharomyces. The topics include central metabolic pathways; catabolism; fermentation; respiration; biosynthesis of small molecules including cofactors; the metabolism of lipids, polysaccharides, and storage molecules; inorganic ions; transport and compartments; the global analysis of metabolism; and issues of metabolic toxicity. The book is expected to be used in courses and as a handbook or reference book for research investigators.

  Publisher

• Will the New York Times Be Next?

  Challenge · 2008-09-01 · 2 citations

  article1st authorCorresponding

  A noneconomist asks a serious question: How can we save the New York Times? Do economists have any serious answers?

  PublisherDOI

• The top genes: on the distance from transcript to function in yeast glycolysis

  Current Opinion in Microbiology · 2003-04-01 · 45 citations

  review1st authorCorresponding
  PublisherDOI

• Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase.

  BMC Genetics · 2002-01-01 · 1 citations

  articleOpen accessSenior author

  BACKGROUND: Setting of graded levels of a protein for in vivo studies by controlled gene expression has inconveniences, and we here explore the use of the t-degron technique instead. RESULTS: In a yeast t-degron (ubiquitin-argDHFR(ts))- phosphoglycerate mutase (GPM1) fusion strain, increasing periods of exposure to the non-permissive temperature 37 degrees C, even in the presence of cycloheximide, gave decreasing function, as assessed at 23 degrees C in vivo by glucose metabolism and confirmed by immunoblot. CONCLUSION: An ideal system would set a range of lower levels of a protein, do so without compensating protein synthesis, and give stable activity for in vitro comparisons. Although the first two aims appear obtainable, the third was not in this example of the application, limiting its uses for some but not all purposes.

  PublisherDOI

• Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase

  BMC Genetics · 2002-07-30

  articleOpen accessSenior author

  Setting of graded levels of a protein for in vivo studies by controlled gene expression has inconveniences, and we here explore the use of the t-degron technique instead. In a yeast t-degron (ubiquitin-argDHFRts)- phosphoglycerate mutase (GPM1) fusion strain, increasing periods of exposure to the non-permissive temperature 37°C, even in the presence of cycloheximide, gave decreasing function, as assessed at 23°C in vivo by glucose metabolism and confirmed by immunoblot. An ideal system would set a range of lower levels of a protein, do so without compensating protein synthesis, and give stable activity for in vitro comparisons. Although the first two aims appear obtainable, the third was not in this example of the application, limiting its uses for some but not all purposes.

  PublisherOA PDFDOI

• Glucose Metabolism in <i>gcr</i> Mutants of <i>Saccharomyces cerevisiae</i>

  Journal of Bacteriology · 2000-04-15 · 2 citations

  articleSenior author
  PublisherDOI

Recent grants

• NIH Grant R01GM021098

  NIH · $2.4M · 1994

Frequent coauthors

• Jorge Babul

  University of Chile

  20 shared

• John M. Sedivy

  Brown University

  18 shared

• B.L. Horecker

  11 shared

• D Clifton

  Sul Ross State University

  7 shared

• Linda F. Bisson

  University of California, Davis

  6 shared

• R T Vinopal

  University of Connecticut

  5 shared

• Drago Clifton

  4 shared

• S. Pontremoli

  PHARM (Italy)

  4 shared

Labs

• Harvard’s Biochemical Sciences Tutorial ProgramPI