Dna-Methylation Eraser Tet2 Activates Wtip Expression to Suppress an Akt-Dependent Chemoresistance of Gastric Cancer

SSRN Electronic Journal · 2025-01-01

Potential Mechanisms Underlying Kaempferol-Promoted Osteoblast Proliferation and Osteogenic Differentiation: A Network Pharmacology and Experimental Validation Study

bioRxiv (Cold Spring Harbor Laboratory) · 2025-04-07

Abstract Background Osteoporosis (OP) manifests primarily in middle-aged and elderly individuals, representing an age-related condition characterised by diminished bone mass and alterations in bone tissue structure, potentially resulting in fractures and compromising the patient’s quality of life. The potential of kaempferol to modulate osteogenic differentiation, enhance bone metabolism, and potentially offer therapeutic benefits in OP cases is of particular interest. This study, employing a combination of network pharmacology and experimental verification, investigated the underlying mechanisms by which kaempferol stimulates the proliferation and osteogenic differentiation of mouse embryonic osteoblast precursor cells MC3T3-E1 subclone 14 through the PI3K/AKT signalling pathway. The findings provide a rational foundation for the potential of kaempferol to promote osteogenesis and enhance the treatment of OP. Methods The present study identified target genes regulated by kaempferol during osteogenesis and differentiation using network pharmacology. To this end, a protein-protein interaction (PPI) network was constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and molecular docking were performed.The cytotoxicity of kaempferol was assessed using the CCK-8 method and the cell clone method in the cell detection section.The macroscopic regulatory mechanism of kaempferol in osteogenic differentiation was studied using alkaline phosphatase staining, activity assay, Alizarin Red staining, and calcium quantification º Furthermore, real-time quantitative polymerase chain reaction (RT qPCR) and Western blot methods were employed to detect the microscopic expression differences of mRNA and protein related to the PI3K/AKT signalling pathway.The present study identified target genes regulated by kaempferol during osteogenesis and differentiation using network pharmacology. To this end, a protein-protein interaction (PPI) network was constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and molecular docking were performed. Results A total of 203 target genes regulated by kaempferol were identified during osteogenic differentiation, with the majority of these genes being associated with biological processes related to cell proliferation and regulation.Four of these target genes act on the PI3K/AKT signalling pathway and show good interactions with kaempferol. Furthermore, kaempferol (5 μM, 10 μM) has been shown to enhance the vitality and proliferation of MC3T3-E1 Subclone 14 cells, as well as to increase alkaline phosphatase activity and calcium deposition. Furthermore, kaempferol (5 μM, 10 μM) has been observed to upregulate the mRNA expression of phosphoinositide 3-kinase (Pi3k), β-catenin, Myc proto-oncogene protein (c-Myc), and cyclin D1 in MC3T3-E1 Subclone. 14 cells, and promotes the phosphorylation of PI3K, serine/threonine protein kinase AKT (AKT1) and glycogen synthase kinase, as well as the phosphorylation of glycogen synthase kinase-3β (GSK3β) (p < 0.05), thereby upregulating the expression of β-catenin, C-MYC and CYCLIN D1 proteins and increasing the levels of p-PI3K/PI3K, p-AKT1/AKT1 and p-GSK3β/GSK3β levels, thereby promoting osteogenic differentiation of MC3T3-E1 Subclone 14. Conclusion Kaempferol has been demonstrated to have the capacity to significantly promote the osteogenic differentiation of MC3T3-E1 Subclone 14. This process is thought to be achieved by regulating the PI3K/AKT signalling pathway and affecting the expression of osteogenic-related genes. It has been shown to have a preventive and therapeutic effect on the occurrence and development of osteoporosis.

Rabbit monoclonal antibodies: Synergistic innovation and breakthrough based on B-cell development mechanism and single B-cell technology

Colloids and Surfaces B Biointerfaces · 2025-10-04 · 1 citations

High-entropy strategy enabling high-reversibility and long-life O3-type oxide cathode

Journal of Energy Storage · 2025-08-13

Preliminary study on the neutron radiography of spent fuel sample from nuclear reactor

Nuclear Techniques · 2025-10-01

<bold>Background</bold>The non-destructive examination of internal structure of spent fuel rod is a very important way to evaluate the performance of spent fuel rods from nuclear reactor after irradiation. Compared with other examination methods, neutron radiography technology has more advantages on the irradiated nuclear fuel with high activity, such as strong penetration, high image sensitivity, and indirect radiography that can avoid radiation damage to radiography components.<bold>Purpose</bold>This study aims to develop and evaluate neutron radiography methods for examining internal structures of highly radioactive spent fuel rods, comparing the advantages and limitations of direct and indirect radiography approaches.<bold>Methods</bold>Firstly, a spent fuel sample transfer and neutron radiography device was designed to satisfy the radiation protection requirements and located at the neutron radiography facility of the China Advanced Research Reactor (CARR). Then, non-destructive testing experiments using both neutron imaging plate (NIP) for direct neutron radiography and neutron conversion screen plus imaging plate (IP) for indirect neutron radiography were carried out on fuel rod simulations with artificial defects and real spent fuel samples using indirect radiography and direct radiography methods respectively under the experimental conditions of neutron flux rate of 1×10⁸ n·cm^-2·s^-1 and neutron beam collimation ratio of 63. Finally, the advantages and disadvantages of the two radiography methods were compared by analyzing the internal structure images of radioactive spent fuel samples from a domestic commercial nuclear power plant.<bold>Results</bold>Experimental results show that both radiography methods obtain the internal structure images of simulated samples and small-sized spent fuel samples. Both methods detect gaps as small as 0.2 mm between pellets whilst direct radiography using NIP plate shows higher grayscale fluctuations (3107-8899) compared to indirect radiography (953-3879), indicating that direct radiography has higher image clarity. However, the coefficient of variation (COV) value of direct photography (0.032 7) is significantly higher than indirect radiography (0.007 4), indicating that indirect radiography has less noise.<bold>Conclusions</bold>Results of this study demonstrate that the direct radiography method using NIP plate has high test efficiency and high clarity, but the signal-to-noise ratio (SNR) is inferior to that of the indirect radiography method. The image of the indirect radiography method is not affected by gamma rays and has a good SNR, but the operation steps are complicated and requires the bonding of the metal conversion screen and the IP plate.

Antiferromagnetic structure of <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow> <mml:msub> <mml:mi>KV</mml:mi> <mml:mn>2</mml:mn> </mml:msub> <mml:msub> <mml:mi>Se</mml:mi> <mml:mn>2</mml:mn> </mml:msub> <mml:mi mathvariant="normal">O</mml:mi> </mml:mrow> </mml:math> : A neutron diffraction study

Physical review. B./Physical review. B · 2025-11-12 · 1 citations

Altermagnetism has been proposed as a distinct class of antiferromagnets exhibiting momentum-dependent spin splitting band structures without requiring spin-orbit coupling. Recently, ${\mathrm{KV}}_{2}{\mathrm{Se}}_{2}\mathrm{O}$ has been identified as a metallic room-temperature altermagnet with $d$-wave spin-momentum locking. Here, we investigate the magnetic structure of ${\mathrm{KV}}_{2}{\mathrm{Se}}_{2}\mathrm{O}$ in both polycrystalline and single-crystal samples using neutron diffraction techniques. The system exhibits G-type antiferromagnetic structure with a N\'eel temperature ${T}_{N}\ensuremath{\approx}$ 400 K. Notably, substantial broadening of magnetic peaks was observed along $L$ at low temperatures in single crystals, consistent with the coexistence of G-type AFM order and a $c$-axis spin density wave. These results demonstrate that bulk ${\mathrm{KV}}_{2}{\mathrm{Se}}_{2}\mathrm{O}$ cannot host altermagnetism.

Magnetic and Electrical Transport Properties of Kagome Ferromagnet Mn4.2ga2.47sn0.33 Single Crystal

SSRN Electronic Journal · 2025-01-01

Magnetic and electrical transport properties of kagome ferromagnet Mn4.2Ga2.47Sn0.33 single crystal

Journal of Alloys and Compounds · 2025-08-11

Distribution and Evolution of Residual Stress in High-Speed Train Wheel by Diffraction Method

SSRN Electronic Journal · 2025-01-01

[Corrigendum] miR‑335 promotes ferroptosis by targeting ferritin heavy chain 1 in in vivo and in vitro models of Parkinson's disease

International Journal of Molecular Medicine · 2025-06-12

Subsequently to the publication of the above paper, an interested reader drew to the authors' attention that, for the JC‑1 staining experiments shown in Fig. 5F on p. 9, the ‘mimic NC’ and ‘inhibitor’ panels appeared to show the same cells, even though the green/red ratio was different comparing between the two data panels. In addition, two further instances of duplicated data panels were identified in Figs. S2 and S3, such that data that were allegedly obtained under different experimental conditions appeared to have been derived from the same original sources. After having asked the authors to explain the errors that had occurred in assembling these figures, they responded to explain that the error in Fig. 5F resulted from the incorrect selection of source images for the channels during the image merging process, whereas the errors in Figs. S2 and S3 occurred due to mistakes made in the naming and management of image files during storage. This led to the unintentional use of incorrect images during the process of figure assembly. Moreover, the authors were able to present to the Editorial Office the original data from the JC‑1 staining experiments belonging to Fig. 5F. The Editor of <em>International Journal of Molecular Medicine</em> has agreed that a corrigendum may be published to account for the errors made in assembling Figs. 5, S2 and S3, and the corrected versions of these figures are shown on the next page. All the authors agree with the publication of this corrigendum, and are thankful to the Editor for giving them the opportunity to present this; moreover, the Editor and the authors apologize to the readership for any inconvenience caused. [International Journal of Molecular Medicine 47: 61, 2021; DOI: 10.3892/ijmm.2021.4894]