About

Dr. Evelyne Kalyoussef is an Associate Professor and the Director of Comprehensive Otolaryngology-Head & Neck Surgery at Rutgers New Jersey Medical School. She joined the faculty of the Department of Otolaryngology-Head and Neck Surgery in August 2012 as an academic general otolaryngologist, caring for both adult and pediatric patients. Dr. Kalyoussef specializes in a broad spectrum of otolaryngologic conditions, including Thyroid and Parathyroid Surgery, Sialendoscopy, Transoral Robotic Surgery, Head and Neck Oncology, Sleep Disorders, and Voice Disorders. She has a particular interest in cancer survivorship care and minimally invasive surgical techniques. Dr. Kalyoussef received her M.D. from the University of Medicine and Dentistry of New Jersey's Robert Wood Johnson Medical School in 2007 and holds a B.A. from Rutgers University. She is fluent in Spanish and Arabic and is committed to providing outstanding patient care, as well as timely communication with referring physicians.

Research topics

• Internal medicine
• Medicine
• Surgery
• Environmental health
• Psychiatry
• Pediatrics
• Virology

Selected publications

• Exploring the Predictive Value of National Residency Matching Program (NRMP) Rank in Residency Outcomes

  Annals of Otology Rhinology & Laryngology · 2026-04-06

  article

  PURPOSE: To examine the relationship between the National Residency Matching Program (NRMP) rank list position and otolaryngology residency performance. METHODS: Five consecutive graduating classes at a single institution's otolaryngology residency training program (2021-2025) were evaluated (n = 18) using their complete 5-year data. Correlation coefficients were calculated to assess the relationship between NRMP rank and in-service exam scores, research productivity, total case logs, milestone evaluations, selection as Administrative Chief Resident, receipt of teaching awards, and post residency position. Milestone evaluations were based on faculty ratings across all 7 core competencies on a 1- to 7-point scale, with an average score calculated for each resident. Spearman's rho was used to evaluate correlations between NRMP rank and both in-service scores and milestone evaluations. Chi-square tests were conducted to assess associations between NRMP rank and categorical outcomes, including selection as Administrative Chief Resident, receipt of the teaching award, and post residency position. RESULTS: = .020). However, no significant correlations between NRMP rank and in-service exam scores, research productivity, total case logs, selection as Administrative Chief Resident, receipt of the teaching award, or post residency position were found. CONCLUSION: Our results suggest that better-ranked residents receive higher milestone evaluation scores. However, NRMP rank alone may not reliably predict performance on in-service exam scores, chief resident selection, receipt of teaching awards, or position after residency training.

  PublisherDOI

• CD9 Identifies and Regulates a Highly Functional T Follicular Helper (Tfh) Cell Subset Necessary for Promoting Antibodies During Infection

  SSRN Electronic Journal · 2025-01-01

  preprintOpen access
  PublisherDOI

• Effects of Maackia amurensis seed lectin (MASL) on OSCC cell morphology, PDPN expression, growth, and motility in a phase 1 clinical trial

  Journal of Cancer Research and Clinical Oncology · 2025-07-19

  articleOpen access

  BACKGROUND: Podoplanin (PDPN) has emerged as a functionally relevant biomarker and chemotherapeutic target expressed by OSCC cells. PDPN signaling can directly increase tumor cell invasion and metastasis, and also inhibit host lymphocyte activation and immune response. Accordingly, antibodies and Maackia amurensis seed lectin (MASL) can target the PDPN receptor to inhibit OSCC cell migration and viability. However, the effects of MASL on OSCC cells in oral cancer patients has not yet been reported. METHODS: We conducted a Phase 1 human clinical trial to examine the effects of a single 100 mg oral dose of MASL on OSCC cell morphology, PDPN expression, and immune cell infiltration in lesions in oral cancer patients. We also examined the effects of MASL on the PDPN expression, motility, and viability of cells cultured from these patient lesions. In addition, we examined the ability of antibodies to target PDPN and kill OSCC cells by near-infrared photoimmunotherapy. RESULTS: MASL administration was found to be safe and did not produce any adverse effects in any patients. While this single dose did not affect OSCC cell morphology in lesions in situ, it did appear to increase lymphocyte infiltration into tumor fields in one patient by over 5 fold (p < 0.01). In addition, MASL inhibited the growth and motility of all OSCC cells cultured from these patient lesions in a dose responsive manner in vitro (p < 0.05 in all cases) We also report that antibodies can target PDPN on OSCC cells obtained from these patients to destroy them by near-infrared photoimmunotherapy (NIR-PIT). CONCLUSION: These results suggest that protocols using MASL and photoimmunotherapies that target PDPN can be developed to effectively treat OSCC lesions in oral cancer patients.

  PublisherDOI

• Anesthetic Challenges of Caring for an Adult With Moebius Syndrome: A Case Report

  Case Reports in Anesthesiology · 2025-01-01

  articleOpen access

  Background: Moebius syndrome (MBS) is a rare congenital disorder with facial nerve palsies and craniofacial malformations, increasing the risk of a difficult airway during anesthesia. We report a difficult airway in an adult with MBS during an elective procedure, which is unique as most reports of MBS are in children. Case Presentation: Our patient was a 30-year-old male diagnosed with MBS at birth and presented with craniofacial and orthopedic malformations, intellectual disability, and facial nerve palsies. Although our patient had prior successful intubations, their airway exam deteriorated from Mallampati Classes II to IV after many surgeries. After fiberoptic intubation with nebulized lidocaine to maintain spontaneous ventilation failed, an attempt with dexmedetomidine and video laryngoscopy was successful. Conclusion: Our case demonstrates the necessity of preparing for a difficult airway and strategies for airway management in an adult with MBS.

  PublisherOA PDFDOI

• TLR10 (CD290) Is a Regulator of Immune Responses in Human Plasmacytoid Dendritic Cells

  The Journal of Immunology · 2024-07-12 · 6 citations

  articleOpen access

  TLRs are the most thoroughly studied group of pattern-recognition receptors that play a central role in innate immunity. Among them, TLR10 (CD290) remains the only TLR family member without a known ligand and clearly defined functions. One major impediment to studying TLR10 is its absence in mice. A recent study on TLR10 knock-in mice demonstrated its intrinsic inhibitory role in B cells, indicating that TLR10 is a potential drug target in autoimmune diseases. In this study, we interrogated the expression and function of TLR10 in human plasmacytoid dendritic cells (pDCs). We have seen that primary human pDCs, B cells, and monocytes constitutively express TLR10. Upon preincubation with an anti-TLR10 Ab, production of cytokines in pDCs was downregulated in response to stimulation with DNA and RNA viruses. Upon further investigation into the possible mechanism, we documented phosphorylation of STAT3 upon Ab-mediated engagement of TLR10. This leads to the induction of inhibitory molecule suppressor of cytokine signaling 3 (SOCS3) expression. We have also documented the inhibition of nuclear translocation of transcription factor IFN regulatory factor 7 (IRF7) in pDCs following TLR10 engagement. Our data provide the (to our knowledge) first evidence that TLR10 is constitutively expressed on the surface of human pDCs and works as a regulator of their innate response. Our findings indicate the potential of harnessing the function of pDCs by Ab-mediated targeting of TLR10 that may open a new therapeutic avenue for autoimmune disorders.

  PublisherOA PDFDOI

• Maackia amurensis seed lectin (MASL) and soluble human podoplanin (shPDPN) sequence analysis and effects on human oral squamous cell carcinoma (OSCC) cell migration and viability

  Biochemical and Biophysical Research Communications · 2024-04-03 · 2 citations

  articleOpen access

  Maackia amurensis lectins serve as research and botanical agents that bind to sialic residues on proteins. For example, M. amurensis seed lectin (MASL) targets the sialic acid modified podoplanin (PDPN) receptor to suppress arthritic chondrocyte inflammation, and inhibit tumor cell growth and motility. However, M. amurensis lectin nomenclature and composition are not clearly defined. Here, we sought to definitively characterize MASL and its effects on tumor cell behavior. We utilized SDS-PAGE and LC-MS/MS to find that M. amurensis lectins can be divided into two groups. MASL is a member of one group which is composed of subunits that form dimers, evidently mediated by a cysteine residue in the carboxy region of the protein. In contrast to MASL, members of the other group do not dimerize under nonreducing conditions. These data also indicate that MASL is composed of 4 isoforms with an identical amino acid sequence, but unique glycosylation sites. We also produced a novel recombinant soluble human PDPN receptor (shPDPN) with 17 threonine residues glycosylated with sialic acid moieties with potential to act as a ligand trap that inhibits OSCC cell growth and motility. In addition, we report here that MASL targets PDPN with very strong binding kinetics in the nanomolar range. Moreover, we confirm that MASL can inhibit the growth and motility of human oral squamous cell carcinoma (OSCC) cells that express the PDPN receptor. Taken together, these data characterize M. amurensis lectins into two major groups based on their intrinsic properties, clarify the composition of MASL and its subunit isoform sequence and glycosylation sites, define sialic acid modifications on the PDPN receptor and its ability to act as a ligand trap, quantitate MASL binding to PDPN with KD in the nanomolar range, and verify the ability of MASL to serve as a potential anticancer agent.

  PublisherDOI

• Anesthetic Challenges of Caring for an Adult with Moebius Syndrome: A Case Report

  Research Square · 2024-07-31 · 1 citations

  preprintOpen access
  PublisherOA PDFDOI

• Human Plasmacytoid Dendritic Cells Express C-Type Lectin Receptors and Attach and Respond to <i>Aspergillus fumigatus</i>

  The Journal of Immunology · 2022-08-01 · 8 citations

  articleOpen access

  Plasmacytoid dendritic cells (pDCs) have been implicated as having a role in antifungal immunity, but mechanisms of their interaction with fungi and the resulting cellular responses are not well understood. In this study, we identify the direct and indirect biological response of human pDCs to the fungal pathogen Aspergillus fumigatus and characterize the expression and regulation of antifungal receptors on the pDC surface. Results indicate pDCs do not phagocytose Aspergillus conidia, but instead bind hyphal surfaces and undergo activation and maturation via the upregulation of costimulatory and maturation markers. Measuring the expression of C-type lectin receptors dectin-1, dectin-2, dectin-3, and mannose receptor on human pDCs revealed intermediate expression of each receptor compared with monocytes. The specific dectin-1 agonist curdlan induced pDC activation and maturation in a cell-intrinsic and cell-extrinsic manner. The indirect activation of pDCs by curdlan was much stronger than direct stimulation and was mediated through cytokine production by other PBMCs. Overall, our data indicate pDCs express various C-type lectin receptors, recognize and respond to Aspergillus hyphal Ag, and serve as immune enhancers or modulators in the overarching fungal immune response.

  PublisherOA PDFDOI

• Multidisciplinary Management of Nasal Dermoid with Intracranial Extension

  Neurology and Neuroscience · 2022-09-30

  articleOpen access

  Nasal dermoids are rare congenital lesions present in 1/20,000 to 1/40,000 patients. Only about 10% of these congenital lesions have an associated tract or tail with an intracranial extension. The intracranial component of the mass predisposes the child to risks of meningitis and abscess formation. We aim to discuss the etiology of dermoid cysts as well as neurosurgical approaches for the management of cases with intracranial extension, which include both endoscopic and open surgical approaches. We present an 18-month-old boy who underwent a bifrontal craniotomy and resection of his nasal dermoid with intracranial extension. This case report and review of the literature exemplify the collaboration between pediatric otorhinolaryngologists and neurosurgeons to successfully remove nasal dermoids specifically presenting with intracranial extensions.

  PublisherOA PDFDOI

• Helping Children with Special Needs: Who Receives Tympanostomy Tubes?

  Annals of Otology Rhinology & Laryngology · 2021-01-16 · 3 citations

  articleSenior authorCorresponding

  Objectives: Tympanostomy tubes can prevent sequelae of otitis media that adversely affect long term hearing and language development in children. These negative outcomes compound the existing difficulties faced by children who are already diagnosed with developmental disorders. This study aims to characterize this subset of children with developmental disorders undergoing myringotomy and tympanostomy tube insertion. Methods: A retrospective review using the Kids’ Inpatient Database (KID) was conducted, with codes from International Classification of Diseases, Ninth Revision used to query data from the years 2003 to 2012 to determine a study group of children with a diagnosis of a developmental disorder undergoing myringotomy and tympanostomy insertion. This group was compared statistically to patients undergoing these procedures who did not have a diagnosed developmental disorder. Results: In total, 21 945 cases of patients with myringotomy with or without tympanostomy tube insertion were identified, of which 1200 (5.5%) had a diagnosis of a developmental disorder. Children with developmental disorders had a higher mean age (3.3 years vs 2.9 years, P = .002) and higher mean hospital charges ($43 704.77 vs $32 764.22, P = .003). This cohort also had higher proportions of black (17.6% vs 12.3%, P &lt; .001) and Hispanic (23.9% vs 20.6%, P = .014) patients, and had lower rates of private insurance coverage (39.6% vs 49%, P &lt; .001). Conclusion: The population of children with developmental disorders undergoing myringotomy or tympanostomy tube placement has a different demographic composition than the general population and faces distinct financial and insurance coverage burdens. Further study should be done to assess if these differences impact long term outcomes.

  PublisherDOI

Frequent coauthors

• Simrandeep Singh

  Guru Gobind Singh Medical College and Hospital

  104 shared

• Marylise Boutros

  Cleveland Clinic Florida

  78 shared

• Lorenzo Conti

  University of Pisa

  78 shared

• H Salem

  75 shared

• Sana Ali

  Allama Iqbal Open University

  75 shared

• Sudha Sundar

  University of Birmingham

  69 shared

• Soly Baredes

  Rutgers, The State University of New Jersey

  63 shared

• Aneel Bhangu

  National Institute for Health Research

  56 shared

Education

• M.D.

  University of Medicine and Dentistry of New Jersey's Robert Wood Johnson Medical School

  2007

• B.A.

  Rutgers University

  2003