About

Amy L. Halverson, MD, is a Professor of Surgery specializing in Gastrointestinal and Endocrine surgery at Northwestern University Feinberg School of Medicine. She serves as Vice Chair of Surgical Education, contributing to the development and oversight of surgical training programs and educational initiatives within the department. Her role involves fostering surgical education and ensuring the quality of training for medical students and residents.

Research topics

• Medicine
• Internal medicine
• Biology
• Immunology
• Political Science
• Computer Science
• Surgery
• Medical education
• Genetics
• Nursing
• Physics
• Intensive care medicine
• Chemistry
• Cell biology
• Physical therapy
• Emergency medicine
• Psychology
• Cancer research
• General surgery
• Pedagogy
• Microbiology
• Pediatrics

Selected publications

• Fielding an Expeditionary Prolonged Casualty Care Kit: What We Carry Matters

  Military Medicine · 2025-06-12

  articleSenior author

  INTRODUCTION: Prolonged Casualty Care (PCC) is an extension of Tactical Combat Casualty Care (TCCC), which provides prehospital care when evacuation is delayed and care is resource constrained and suboptimal. Current medical rucksacks are designed to maximize medical equipment, without sustainment items like food, clothing, and essential communication equipment. This leaves limited room for PCC expendable supplies and equipment to expand upon existing TCCC loadouts. The multi-day premise of PCC requires both extended medical and general survival items, for which existing medical kits are poorly suited and not optimized. In this study, we sought to systematically create a Prolonged Field Care Kit (PFAK) capable of providing field medics with medications and equipment adjuncts for PCC as well as a specialized long-range medical rucksack (LMR) to house the PFAK, TCCC, and sustainment items. MATERIALS AND METHODS: Baseline design elements from conventional rucksacks were obtained and modified to address known limitations. Initial prototypes for the PFAK and LMR were created. These were modified following informal discussions with various and multiple end users from both U.S. conventional and Special Operations Forces. Feedback regarding the second iteration prototype was obtained systematically through written surveys regarding strengths, weaknesses, and potential changes that could be made. Qualitative data were analyzed using MAXQDA software. RESULTS: Using informal feedback, we deduced that the first PFAK prototype lacked internal compartmentalization and could not accommodate the volume of intravenous fluid needed for PCC. Moreover, the LMR was prone to damage by external factors such as rainwater. The second prototype addressed these flaws and written feedback was obtained from medics (n = 10), nurses (n = 11), and medical doctors (n = 3) on its applicability. Positive feedback received on the second prototype included daypack detachability, overall organization, versatility, and design of the LMR and PFAK. However, most survey participants felt that the LMR was too complex and that its heaviness could limit its field use. After the feedback obtained, the finalized PFAK weighed 18 pounds and had a volume of 30 L. The LMR containing the PFAK and other mixed sustainment and TCCC equipment has a length of 30 inches and weighs 110 pounds with all items intact. CONCLUSIONS: PCC will require thoughtful equipping to enable prehospital providers to manage multiple, potentially complex casualties; existing military medical backpacks are not currently optimized for this scenario. Our group created the first-of-its-kind PFAK, as well as the LMR to house it. The final prototypes of our LMR and PFAK are versatile, well organized, and significantly improve upon current backpack options for PCC. However, field testing and further improvement through qualitative analysis are needed.

  PublisherDOI

• 2540eTiP FLEXible and extended dosing of 177Lu-PSMA-617 molecular radioligand therapy in mCRPC (FLEX-MRT): A randomized phase II trial in progress

  Annals of Oncology · 2025-09-01

  article
  PublisherDOI

• Genomic analysis of surgical patients to identify patients at risk for postoperative sepsis and surgical site infection

  The Journal of Trauma: Injury, Infection, and Critical Care · 2025-01-06

  article

  BACKGROUND: Early and accurate diagnosis of sepsis and the ensuing organ dysfunction remain a challenge in the postoperative setting. Susceptibility to infections, as well as the subsequent immunological response, are driven to some extent by the genetic predisposition of the patient. The purpose of this study was to identify novel genetic variants associated with postoperative sepsis (POS) and surgical site infections (SSIs). METHODS: We conducted genome-wide association studies for POS and SSIs in the Electronic Medical Records and Genomics Network database. All patients with surgical and genomic information in Electronic Medical Records and Genomics were identified. Patients with a new diagnosis of sepsis/SSIs after surgery were classified as cases, and those without as controls. Analyses were performed using PLINK 2.0's logistic regression function. A p value of <5 × 10 -8 was considered statistically significant. RESULTS: A total of 59,755 participants were included in the analysis. Genetic regions on chromosomes 9 and 14 reached statistical significance for POS ( p < 5 × 10 -8 ). The most significant single-nucleotide polymorphisms (SNPs) were rs9413988 ( p = 5.59 × 10 -12 ) on chromosome 9 and rs35407594 ( p = 1.43 × 10 -10 ) on chromosome 14. The rs9413988 region is downstream to the phosphoglucomutase 5 pseudogene ( PGM5P2 ) and Zn-regulated GTPase metalloprotein activator 1F ( ZNGF1 ) and likely plays a role in transcription regulation, while rs35407594 corresponds to the olfactory receptor gene family, OR11 . Similar SNPs were also associated with SSIs. CONCLUSION: We have identified two genetic regions containing SNPs associated with POS and SSIs. These findings provide new avenues for investigation, which may help identify and guide point-of-care management for at-risk patients. LEVEL OF EVIDENCE: Prognostic and Epidemiological; Level III.

  PublisherDOI

• Tranexamic Acid in General Surgery—Who Benefits the Most?

  JAMA Surgery · 2025-01-15

  articleSenior author
  PublisherDOI

• Farmer field school approach for scaling experimental lily-based cropping systems in tidal coastal belts

  International Journal of Agriculture Extension and Social Development · 2025-10-01

  articleOpen accessSenior author

  Tidal coastal belts in South Asia, especially the Sundarbans and other low-lying deltaic regions, represent some of the most ecologically fragile and agriculturally challenging landscapes. These areas frequently face tidal flooding, salinity intrusion, waterlogging, cyclonic disturbances, and limited access to freshwater, all of which collectively reduce agricultural productivity and restrict diversification options for smallholder farmers [1-3]. Traditional rice-based systems continue to underperform due to increasing soil salinity, unstable hydrological conditions, and poor soil structure, compelling farmers to explore climate-resilient and economically viable alternatives [4, 5]. Among emerging horticultural options, lily (Lilium spp.) has shown considerable promise. Research indicates that lily cultivation performs well under raised-bed and micro-elevated systems, making it compatible with tidal agro-ecosystems where drainage and salinity management are crucial [6-8]. Experimental evidence from the Sundarbans highlights that optimized lily plantation designs particularly those involving elevated beds, controlled spacing, and improved drainage can enhance bulb development, reduce inundation stress, and yield higher economic returns. A notable study by Das (2023) provides detailed insights into experimental lily plantation design specifically adapted for the Sundarbans environment, demonstrating its feasibility for wider application in coastal systems [9]. Despite such promising results, widespread adoption of lily-based cropping systems remains limited due to knowledge gaps, inadequate extension support, and the absence of participatory mechanisms for farmer learning. The Farmer Field School (FFS) approach has proven to be an effective method for hands-on knowledge dissemination, farmer-led experimentation, and improved decision-making in diverse agro-ecosystems [10-12]. FFS is especially valuable in fragile and heterogeneous environments where adaptive learning, group problem-solving, and real-time observation are essential for technology adoption [13-15]. Integrating the FFS model with lily-based experimental cropping systems could help farmers evaluate performance under different salinity levels, improve planting techniques, and identify suitable raised-bed configurations through collaborative field trials [16-18]. However, there is limited empirical evidence on the effectiveness of FFS in scaling lily-based systems within the unique constraints of tidal coastal belts. The present research investigates the potential of FFS as a platform for disseminating, validating, and scaling experimental lily-based cropping systems in tidal coastal regions. By comparing pre- and post-intervention knowledge levels, crop performance metrics, economic returns, and farmer perceptions, the research aims to determine whether structured experiential learning can accelerate adoption. Findings are expected to generate actionable insights for agricultural extension agencies, coastal development planners, and policymakers. Ultimately, this research contributes to developing climate-resilient diversification strategies that enhance income stability and ecological sustainability in vulnerable coastal farming systems.

  PublisherOA PDFDOI

• Invited Commentary: Bridging Faith and Function: A Seamless Solution for Surgical Inclusivity

  Journal of the American College of Surgeons · 2025-05-23

  articleSenior author
  PublisherDOI

• Development of a prolonged field care kit using a modified Delphi survey approach

  The Journal of Trauma: Injury, Infection, and Critical Care · 2025-04-10

  articleSenior authorCorresponding

  BACKGROUND: Prolonged Casualty Care (PCC) is a military adaptation of Tactical Combat Casualty Care providing up to 72 hours of prehospital care in delayed extrication. However, providing PCC is challenging because of recommended medications outweighing size/weight restrictions during dismounted operations. We sought to narrow down the medication and supply items required for 72 hours of PCC to create a standardized and effective prolonged field care kit (PFAK) with a weight limitation of 20 lbs. METHODS: Joint Trauma Systems Clinical Practice Guidelines were reviewed to generate a list of potential contents for the PFAK. A two-step Delphi survey that included various PCC subgroups was performed. The Delphi results were subjected to an expert panel (containing military and civilian medical personnel) survey to finalize the choices. Participant responses were analyzed using mean rank scores and finalized using expert consensus to determine the final components of the PFAK. RESULTS: Eleven Joint Trauma Systems Clinical Practice Guidelines relevant to PCC were selected, encompassing 47 potential medications and 5 adjuncts to PCC. The Delphi surveys were distributed to 100 participants (response rates of 57% and 65% for the two rounds, respectively). A total of 18 medications were shortlisted after the second Delphi survey. Finally, following the expert panel survey, 14 medications and 4 equipment were shortlisted for the PCC. The total weight of the PFAK with medications and adjuncts was under 20 lbs. CONCLUSION: Providing efficient PCC is limited by the size and weight restrictions in military dismounted operations. Using a Delphi survey approach, we have shortlisted the optimal medications and adjuncts that can be used to deliver effective PCC. LEVEL OF EVIDENCE: Prognostic and Epidemiological; Level IV.

  PublisherDOI

• Prolonged Partial Occlusion of the Aorta in a Swine Model of Hemorrhage and Traumatic Brain Injury Does Not Worsen the Brain Lesion But Significantly Impacts Hemodynamic Stability

  Shock · 2025-05-27

  articleSenior authorCorresponding

  INTRODUCTION: Hemorrhagic shock (HS) and traumatic brain injury (TBI) are leading causes of death in trauma. It has been shown that a novel partially occluding resuscitative endovascular balloon occlusion of the aorta device (p-REBOA) can be deployed in the thoracic aorta for up to 2 h with minimal downstream ischemia. However, the impact of partial occlusion on the injured brain has not been studied. We hypothesized that the use of the p-REBOA in a model of TBI and HS would not worsen the brain lesion size. METHODS: Yorkshire, female swine (40-45 kg; n = 22) were subjected to controlled cortical impact TBI and 40% blood volume loss. After 1 h of shock, they were randomized to either (1) placement of p-REBOA for 2 h (p-REBOA group) or (2) no p-REBOA (control). Brain lesion size, survival rates, resuscitation requirements, and key laboratory values were used to compare the groups. RESULTS: Nineteen animals survived to the end of the experiment, with all three deaths in the p-REBOA group ( P = 0.1336). The brain lesion size was similar between the groups (mean p-REBOA volume vs. control: 3,690.93 ± 1,027.21 mm 3 and 2,961.32 ± 807.31 mm 3 , respectively; P = 0.1245). The p-REBOA group showed more severe tissue ischemia, as defined by worse peak lactic acidosis ( P < 0.000001). All animals in the p-REBOA group required vasopressor support during the critical care period compared to one in the control group ( P = 0.0001). CONCLUSION: Prolonged partial occlusion of the thoracic aorta in a combined model of HS and TBI results in significant hemodynamic instability, without an increase in the brain lesion size.

  PublisherDOI

• A citrullinated histone H3 monoclonal antibody for immune modulation in sepsis

  Nature Communications · 2025-08-12 · 16 citations

  articleOpen access

  Citrullinated histone H3 (CitH3), released from immune cells during early sepsis, drives a vicious cycle of inflammation through excessive NETosis and pyroptosis, causing immune dysfunction and tissue damage. To regulate this process, we develop a humanized CitH3 monoclonal antibody (hCitH3-mAb) with high affinity and specificity to target this process. In murine models, hCitH3-mAb reduces cytokine production, mortality and acute lung injury (ALI) caused by LPS and Pseudomonas aeruginosa while enhancing bacteria phagocytosis in the lungs, spleen, and liver. Using pre-equilibrium digital ELISA (PEdELISA), we identify an optimal therapeutic window for hCitH3-mAb in sepsis-induced ALI. In parallel, we explore the molecular mechanism underlying CitH3-driven inflammation. We find that in macrophages, CitH3 activates Toll-like receptor 2 (TLR2), triggering Ca2+-dependent PAD2 auto-citrullination and nuclear translocation, amplifying CitH3 production via a harmful feedback loop. The hCitH3-mAb treatment effectively disrupts this cycle and restores macrophage function under septic conditions. Together, these findings highlight both the therapeutic potential of hCitH3-mAb and provide a deep mechanistic insight into the CitH3–PAD2 axis in sepsis, supporting its further development for treating immune-mediated diseases. This study reports the development and preclinical evaluation of a humanized citrullinated histone H3 (CitH3) monoclonal antibody that mitigates inflammation, restores macrophage function, and protects against sepsis-induced pulmonary injury.

  PublisherOA PDFDOI

• Treatment with MG53 ameliorates traumatic brain injury–associated acute kidney injury

  The Journal of Trauma: Injury, Infection, and Critical Care · 2025-06-12 · 1 citations

  articleOpen accessSenior authorCorresponding

  INTRODUCTION: Multiorgan dysfunction (MOD) after traumatic brain injury (TBI) results in increased morbidity and mortality. There is emerging evidence demonstrating TBI-induced inflammatory responses; however, the mechanisms driving TBI-induced organ injury remains unknown and understudied. MG53, a cell membrane repair protein, has been shown to reduce brain lesion size following TBI. In this study, we aimed to establish a large animal model of post-TBI MOD, determine MG53's role in renal protection following TBI, and explore a mechanistic link between endothelial cell dysfunction and post-TBI MOD. METHODS: Female Yorkshire swine (n = 5/group) were subjected to controlled cortical impact TBI and randomized to receive (1) MG53 protein therapy or (2) normal saline (control). Biomarkers of acute kidney injury were compared between the groups. Kidneys were analyzed for histologic evidence of acute injury. Top-down proteomics were performed on swine plasma at various times post-TBI. RESULTS: Control animals had a significant increase in creatinine from baseline by 6 hours post-TBI ( p = 0.007), which was attenuated in the MG53-treated animals ( p = 0.089). Control animals had a significant increase in plasma NGAL from baseline starting at 4 hours from baseline ( p = 0.014). Animals treated with MG53 had no change in serum NGAL from baseline ( p = 0.163). Histologic analysis showed protection of proximal tubular epithelial cell damage in animals treated with MG53. Proteoform data showed differential expression of apolipoprotein 1, fibrinogen β, and osteocalcin. CONCLUSION: Traumatic brain injury can induce distant organ damage, possibly through endothelial cell dysfunction, and treatment with a cell membrane repair protein (MG53) can protect against this injury.

  PublisherOA PDFDOI

Recent grants

• Modulation of Acetylation in the Treatment of Lethal Injuries

  NIH · $310k · 2009–2020

• NIH Grant R01HL071698

  NIH · $1.5M · 2007

• Modulation of Acetylation in the Treatment of Lethal Injuries

  NIH · $2.3M · 2009–2020

Frequent coauthors

• Peter Rhee

  Hackensack University Medical Center

  384 shared

• Elena Koustova

  322 shared

• Baoling Liu

  Linyi People's Hospital

  314 shared

• Ernest E. Moore

  University of Colorado Denver

  300 shared

• David V. Shatz

  273 shared

• George C. Velmahos

  Harvard University

  241 shared

• Ben E. Biesterveld

  University of Wisconsin–Madison

  209 shared

• Marc DeMoya

  Medical College of Wisconsin

  204 shared