About

Huiying Chen is an Assistant Professor in the Department of History at Purdue University, affiliated with Asian Studies and the SLC. She earned her Ph.D. at the University of Illinois at Chicago in 2021. Her research specializes in the history of China after 1600, with a methodological focus on the history of cartography, book history, printing, and the early modern world. Prior to joining Purdue, she taught at the University of Maryland, College Park, and has been a Visiting Fellow at Harvard University’s Department of East Asian Languages and Civilizations, as well as a Predoctoral Fellow at the Max Planck Institute for the History of Science in Berlin. Her dissertation, supported by the Mellon Fellowship for Dissertation Research in Original Sources and awarded the Graduate Student Best Paper Prize by the China and Inner Asia Council of the Association for Asian Studies, explores the everyday experience of travel in 17th, 18th, and 19th-century China. She is currently working on a book manuscript on this topic. Her recent publication includes an article titled “The Pragmatic Way: Managing the Streets in Qing Beijing” in the Journal of Urban History.

Research topics

• Pharmacology
• Biology
• Chemistry
• Molecular biology
• Traditional medicine
• Biochemistry
• Medicine

Selected publications

• Global existence and large-time behavior of spherically symmetric solutions for a viscous heat-conducting ionized gas in exterior domains

  Nonlinear Analysis · 2025-12-05

  article1st authorCorresponding
  PublisherDOI

• Essential oil of lemon myrtle (Backhousia citriodora) induces S-phase cell cycle arrest and apoptosis in HepG2 cells

  Journal of Ethnopharmacology · 2023 · 14 citations

  • Biology
  • Pharmacology
  • Molecular biology
  PublisherDOI

• Detection of Neutral CO Lost During Ionic Dissociation Using Atmospheric Pressure Thermal Dissociation Mass Spectrometry (APTD-MS)

  Journal of the American Society for Mass Spectrometry · 2018-09-11 · 5 citations

  articleSenior author

  Elucidation of ion dissociation patterns is particularly important to structural analysis by mass spectrometry (MS). However, typically, only the charged fragments from an ion dissociation event are detected in tandem MS experiments; neutrals are not identified. In recent years, we have developed an atmospheric pressure thermal dissociation (APTD) technique that can be applied to dissociate ions at atmosphere pressure and thus provide one way to characterize neutral fragments. In this paper, we focus on the detection of neutral CO resulting from amino acid and peptide ion dissociation. In the first set of experiments, several protonated amino acids (e.g., + 1 ion of phenylalanine) were found to undergo loss of a neutral (s) of total mass 46 Da, a process leading to iminium ion formation. We successfully detected the neutral species CO by using a CO sensor, UV-Vis and MS analysis following selective CO trapping with a rhodium complex. The capture of CO from dissociation of protonated amino acids supports the assignment of the loss of 46 Da to neutral losses of CO and H2O, rather than loss of formaldehyde or dihydroxycarbene, other possible fragmentation pathways that have been subject of debate for a long time. In a second experiment, we used the APTD method in combination with the CO detection technique, to demonstrate the formation of CO in the conversion of b ions to a ions during peptide ion dissociations. These results showed the potential of APTD in the elucidation of ion dissociation mechanisms, using simple home-built apparatus.

  PublisherDOI

• Fluorescence imaging of selenol in HepG2 cell apoptosis induced by Na₂SeO₃

  Chemical Communications · 2015-01-01 · 59 citations

  article

  A novel fluorescence probe (HB) has been designed and synthesized to image selenol in living cells and in vivo for the first time, and used to investigate the Na2SeO3 anticancer mechanism in HepG2 cells.

  PublisherDOI

• Assembly and Evolution of Low-dimensional Nanomaterials in Silica Aerogel

  2014-01-01

  article
  Publisher

• Nuclear Magnetic Resonance Structure Elucidation of Peptide <i>b₂</i> Ions

  Angewandte Chemie · 2014-12-10 · 4 citations

  articleSenior authorCorresponding

  Abstract Tandem mass spectrometry (MS/MS) is powerful for chemical identification but it is still insufficient for explicit ion structure determination. A strategy is introduced to elucidate MS fragment ion structures using NMR spectroscopy for the first time. In our experiments, precursor ions are dissociated at atmospheric pressure and the resulting fragment ions are identified by mass spectrometry but collected outside the mass spectrometer, making the subsequent NMR measurements possible. This new strategy has been applied to determine the chemical structure of the characteristic b 2 fragment ion, a subject of longstanding debate in MS‐based proteomics.

  PublisherDOI

• Nuclear Magnetic Resonance Structure Elucidation of Peptide <i>b₂</i> Ions

  Angewandte Chemie International Edition · 2014-12-10 · 15 citations

  articleSenior authorCorresponding

  Tandem mass spectrometry (MS/MS) is powerful for chemical identification but it is still insufficient for explicit ion structure determination. A strategy is introduced to elucidate MS fragment ion structures using NMR spectroscopy for the first time. In our experiments, precursor ions are dissociated at atmospheric pressure and the resulting fragment ions are identified by mass spectrometry but collected outside the mass spectrometer, making the subsequent NMR measurements possible. This new strategy has been applied to determine the chemical structure of the characteristic b2 fragment ion, a subject of longstanding debate in MS-based proteomics.

  PublisherDOI

• Tandem MS Analysis of Selenamide-Derivatized Peptide Ions

  Journal of the American Society for Mass Spectrometry · 2011-06-08 · 11 citations

  articleOpen accessSenior author

  Our previous study showed that selenamide reagents such as ebselen and N-(phenylseleno)phthalimide (NPSP) can be used for selective and rapid derivatization of protein/peptide thiols in high conversion yield. This paper reports the systematic investigation of MS/MS dissociation behaviors of selenamide-derivatized peptide ions upon collision induced dissociation (CID) and electron transfer dissociation (ETD). In the positive ion mode, derivatized peptide ions exhibit tag-dependent CID dissociation pathways. For instance, ebselen-derivatized peptide ions preferentially undergo Se-S bond cleavage upon CID to produce a characteristic fragment ion, the protonated ebselen (m/z 276), which allows selective identification of thiol peptides from protein digest as well as selective detection of thiol proteins from protein mixture using precursor ion scan (PIS). In contrast, NPSP-derivatized peptide ions retain their phenylselenenyl tags during CID, which is useful in sequencing peptides and locating cysteine residues. In the negative ion CID mode, both types of tags are preferentially lost via the Se-S cleavage, analogous to the S-S bond cleavage during CID of disulfide-containing peptide anions. In consideration of the convenience in preparing selenamide-derivatized peptides and the similarity of Se-S of the tag to the S-S bond, we also examined ETD of the derivatized peptide ions to probe the mechanism for electron-based ion dissociation. Interestingly, facile cleavage of Se-S bond occurs to the peptide ions carrying either protons or alkali metal ions, while backbone cleavage to form c/z ions is severely inhibited. These results are in agreement with the Utah-Washington mechanism proposed for depicting electron-based ion dissociation processes.

  PublisherOA PDFDOI

• <b>Mass spectrometry instrumentation, interpretation, and applications</b> Rolf Ekman, Jerzy Silberring, Ann M. Westman-Brinkmalm, and Agnieszka Kraj, Editors

  Journal of the American Society for Mass Spectrometry · 2009-10-16 · 1 citations

  article1st authorCorresponding

  ADVERTISEMENT RETURN TO ISSUEPREVBook ReviewMass spectrometry instrumentation, interpretation, and applications Rolf Ekman, Jerzy Silberring, Ann M. Westman-Brinkmalm, and Agnieszka Kraj, EditorsHao ChenHao ChenCenter for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Clippinger Laboratories, Ohio University, 45701, Athens, OH, USAMore by Hao ChenCite this: J Am Soc Mass Spectrom 2010, 21, 1, R1Publication Date (Web):November 22, 2011Publication History Published online22 November 2011Published inissue 1 January 2010https://pubs.acs.org/doi/10.1016/j.jasms.2009.09.012https://doi.org/10.1016/j.jasms.2009.09.012book-reviewACS PublicationsCopyright © 2010 © American Society for Mass Spectrometry 2010Request reuse permissionsArticle Views21Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts

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• In Situ Trace Detection of Peroxide Explosives by Desorption Electrospray Ionization and Desorption Atmospheric Pressure Chemical Ionization

  Analytical Chemistry · 2008-02-05 · 187 citations

  article

  Desorption electrospray ionization (DESI) mass spectrometry is used for the rapid (<5 s), selective, and sensitive detection of trace amounts of the peroxide-based explosives, hexamethylene triperoxide diamine (HMTD), tetracetone tetraperoxide (TrATrP), and triacetone triperoxide (TATP), directly from ambient surfaces without any sample preparation. The analytes are observed as the alkali metal ion complexes. Remarkably, collision-induced dissociation (CID) of the HMTD, TATP, and TrATrP complexes with Na(+), K(+), and Li(+) occurs with retention of the metal, a process triggered by an unusual homolytic cleavage of the peroxide bond, forming a distonic ion. This is followed by elimination of a fragment of 30 mass units, shown to be the expected neutral molecule, formaldehyde, in the case of HMTD, but shown by isotopic labeling experiments to be ethane in the cases of TATP and TrATrP. Density functional theory (DFT) calculations support the suggested fragmentation mechanisms for the complexes. Binding energies of Na+ of 40.2 and 33.1 kcal/mol were calculated for TATP-Na(+) and HMTD-Na(+) complexes, suggesting a strong interaction between the peroxide groups and the sodium ion. Increased selectivity is obtained either by MS/MS or by doping the spray solvent with additives that produce the lithium and potassium complexes of TATP, HMTD, and TrATrP. Addition of dopants into the solvent spray increased the signal intensity by an order of magnitude. When pure alcohol or aqueous hydrogen peroxide was used as the spray solvent, the (HMTD + Na)+ complex was able to bind a molecule of alcohol (methanol or ethanol) or hydrogen peroxide, providing additional characteristic ions to increase the selectivity of analysis. DESI also allowed the rapid detection of peroxide explosives in complex matrixes such as diesel fuel and lubricants using single or multiple cation additives (Na(+), K(+), and Li(+), and NH4(+)) in the spray solvent. Low-nanogram detection limits were achieved for HMTD, TrATrP, and TATP in these complex matrixes. The DESI response was linear over 3 orders of magnitude for HMTD and TATP on paper surfaces (1-5000 ng), and quantification of both peroxide explosives from paper gave precisions (RSD) of less than 3%. The use of pure water and compressed air as the DESI spray solution and nebulizing gas, respectively, showed similar ionization efficiencies to those obtained using methanol/water mixtures and nitrogen gas (the typical choices). An alternative ambient method, desorption atmospheric pressure chemical ionization (DAPCI), was also used to detect trace amounts of HMTD and TATP in air by complexation with gas-phase ammonium ions (NH4(+)) generated by atmospheric pressure ammonia ionization.

  PublisherDOI

Recent grants

• Development of Electrochemical Mass Spectrometry for the Study of Protein Redox Chemistry and Protein Structures

  NSF · $313k · 2018–2021

• The Study of Ion/Ion Reactions at Atmospheric Pressure by Ambient Neutral Re-ionization Mass Spectrometry and Ambient Soft Landing

  NSF · $314k · 2009–2012

• Development of Electrochemical Mass Spectrometry for the Study of Protein Redox Chemistry and Protein Structures

  NSF · $363k · 2017–2019

• CAREER: Development of Microsecond Time-Resolved Mass Spectrometry for the Study of Biochemical Reaction Mechanisms and Kinetics

  NSF · $575k · 2012–2017

Frequent coauthors

• R. Graham Cooks

  Purdue University West Lafayette

  37 shared

• Pengyuan Liu

  12 shared

• Pengyi Zhao

  New Jersey Institute of Technology

  10 shared

• Zheng Ouyang

  5 shared

• Marcos N. Eberlin

  4 shared

• Guangming Huang

  University of Science and Technology of China

  4 shared

• Eduardo C. Meurer

  4 shared

• Barbara M. Hopkins

  4 shared

Awards & honors

• Mellon Fellowship for Dissertation Research in Original Sour…
• Graduate Student Best Paper Prize selected by the China and…