Engineered self-assembly of mucoadhesive Helicobacter pylori vaccines: CD4+ T cell-dependent immunity prevents bacterial colonization

Journal of Controlled Release · 2025-11-27 · 1 citations

Moxifloxacin-Loaded Polymeric Nanoparticles for Overcoming Multidrug Resistance in Chronic Pulmonary Infections Caused by <i>Pseudomonas aeruginosa</i>

ACS Applied Materials & Interfaces · 2025-01-13 · 9 citations

Pseudomonas aeruginosa (P. aeruginosa) infections are increasingly challenging due to their propensity to form biofilms and low outer membrane permeability, especially in chronically infected patients with thick mucus. P. aeruginosa exhibits multiple drug resistance mechanisms, making it one of the most significant global public health threats. In this study, we found that moxifloxacin (MXC) and antibacterial peptides (ε-poly-l-lysine, ε-PLL) exhibited a synergistic effect against multidrug-resistant P. aeruginosa (MDR-P. aeruginosa). MXC was combined with ε-PLL to prepare lipase-responsive nanoparticles (MCIP/(PEG–PCL)/PLL NPs) with a weakly negative charge. The weakly negatively charged MCIP/(PEG–PCL)/PLL NPs demonstrated remarkable mucus and biofilm penetration capabilities, thereby overcoming one of the adaptive drug resistance mechanisms. MCIP/(PEG–PCL)/PLL NPs improved the outer and inner membrane permeability and inhibited the expression of the efflux pump MexAB-OprM gene in MDR-P. aeruginosa, thereby overcoming mechanisms of both intrinsic and acquired drug resistance. Meanwhile, the nanoparticles demonstrated an ability to reduce repeated infections with MDR-P. aeruginosa. Additionally, the bacterial burden in the lungs of mice treated with MCIP/(PEG–PCL)/PLL NPs was significantly lower than that in the MXC group, resulting in a 99% clearance rate. Notably, MCIP/(PEG–PCL)/PLL NPs showed no toxicity toward BEAS-2B cells or RAW 267.4 cells, nor did they adversely affect pulmonary function or major organs. This study demonstrated the potential of the nanodrug delivery system composed of the antibiotic moxifloxacin and the antibacterial peptide ε-PLL in addressing the clinical challenges of treating chronic pulmonary infections caused by MDR-P. aeruginosa.

The enhanced electrical, optical, and stability performance of AZO/Ag/AZO film achieved by replacing Ag with Ag/Al bilayer structure

Journal of Materials Science Materials in Electronics · 2025-09-01

Liposomal Formulation Reduces Transport and Cell Uptake of Colistin in Human Lung Epithelial Calu-3 Cell and 3D Human Lung Primary Tissue Models

AAPS PharmSciTech · 2024-02-17 · 4 citations

Antibacterial activity and mechanism of colistin-loaded polymeric nanoparticles for combating multidrug-resistant Pseudomonas aeruginosa biofilms: A synergistic approach

International Journal of Biological Macromolecules · 2024-10-20 · 5 citations

Dual tobramycin and docosahexaenoic acid loaded nanoemulsions combating Pseudomonas aeruginosa-induced pulmonary infection

Colloids and Surfaces B Biointerfaces · 2024-07-11 · 10 citations

Ultraviolet Positioning via TDOA: Error Analysis and System Prototype

arXiv (Cornell University) · 2024-02-29

This work performs the design, real-time hardware realization, and experimental evaluation of a positioning system by ultra-violet (UV) communication under photon-level signal detection. The positioning is based on time-difference of arrival (TDOA) principle. Time division-based transmission of synchronization sequence from three transmitters with known positions is applied. We investigate the positioning error via decomposing it into two parts, the transmitter-side timing error and the receiver-side synchronization error. The theoretical average error matches well with the simulation results, which indicates that theoretical fitting can provide reliable guidance and prediction for hardware experiments. We also conduct real-time hardware realization of the TDOA-based positioning system using Field Programmable Gate Array (FPGA), which is experimentally evaluated via outdoor experiments. Experimental results match well with the theoretical and simulation results.

MgO templated carbons: Effect of synthesis methodology on the morphology, surface chemistry and supercapacitor performances

Diamond and Related Materials · 2024-03-28 · 5 citations

Resveratrol-Loaded Versatile Nanovesicle for Alopecia Therapy via Comprehensive Strategies

International Journal of Nanomedicine · 2024-12-01 · 5 citations

Introduction: Alopecia is a systemic disease with multiple contributing factors. Effective treatment is challenging when only hair growth mechanisms are targeted while ignoring the role of maintaining hair follicle microenvironment homeostasis, which is crucial for cell growth and angiogenesis. Oxidative stress and inflammation are major disruptors of this microenvironment, leading to inhibited cell proliferation and compromised hair follicle circulation. Drugs with antioxidant and anti-inflammatory effects could potentially restore microenvironment homeostasis, offering a promising strategy for alopecia treatment. Methods: Resveratrol (RES), a potent antioxidant and anti-inflammatory agent, was selected as the model drug and encapsulated into an active carrier-PPD-Lip to create PPD-Lip@RES. The efficacy of PPD-Lip@RES was comprehensively evaluated in both in vitro and in vivo aspects, and its underlying mechanism was also primarily explored. Results: PPD-Lip@RES promoted the proliferation and migration of dermal papilla cells, up-regulated the expression of positive hair growth regulators, and facilitated angiogenesis. It also activated hair follicle stem cells by increasing the expression of Ki67, K5, β-catenin, CD31, and CK19. In the telogen effluvium model, PPD-Lip@RES resulted in more robust hair regeneration, with less hair shedding compared to the minoxidil group. Furthermore, it showed significant therapeutic effects in severe androgenetic alopecia, outperforming finasteride and even the healthy control group. Conclusion: The results suggested that PPD-Lip@RES, as a systemic intervention strategy, could effectively facilitate hair growth by targeting both the pathological and physiological processes involved in hair loss. Its superior performance in both telogen effluvium and androgenetic alopecia models indicates its potential as an advanced treatment option for alopecia.

Tailored multilayer nanoparticles against resistant P. aeruginosa by disrupting the thickened mucus, dense biofilm and hyperinflammation

Journal of Controlled Release · 2024-12-25 · 12 citations